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Buffer Recipe Calculator

Pick a buffer system and target pH to calculate the volumes of acid and conjugate base needed, using the Henderson-Hasselbalch equation.

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Example

Buffer Recipe Calculator

Sample: acetate buffer, pH 4.76, 1 L

Example shown — replace it with your own buffer system and target pH.

Target pH

4.76

Volume

1 L, 0.1 M

Result

50 mL acetic acid + 50 mL sodium acetate (1:1 ratio, pKa 4.76)

How it works

  1. Pick your buffer system and target pH.
  2. Enter your desired volume and concentration.
  3. Get the volumes of acid and conjugate base to mix.

The method

The Henderson-Hasselbalch equation calculates the acid-to-base ratio needed for your target pH, based on the buffer system's known pKa.

FAQ

What formula does this use?

The Henderson-Hasselbalch equation, which relates pH to the ratio of a buffer's conjugate base and weak acid concentrations based on the system's pKa.

Why is the target pH close to the pKa?

A buffer works best (resists pH change most effectively) when the target pH is within about 1 unit of the pKa, since that's where the acid/base ratio stays balanced enough to absorb added acid or base.

Does this account for ionic strength or temperature effects?

No — this gives a standard Henderson-Hasselbalch estimate; for precision work, verify the final pH with a calibrated meter after mixing.

How we compare

FeatureOnline Tool StoreManual Henderson-Hasselbalch calculationLab buffer prep software
No software installYesYesNo
Quick to startYesSlowSetup required
Free to useYesFreeOften paid

For a quick prep calculation without opening lab software, this saves working the equation by hand each time.

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